FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5410133
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA24250.028.7998162
CGAGTAG24300.028.7405593
AGTAGCT84750.026.5909795
GCTGGGA181300.025.4162759
GAGTAGC53800.025.159694
TTGGGAG129000.024.7812356
GTAGCTG92100.024.4688996
TAGCTGG103150.022.6852657
AAGTGCT109250.021.9458565
CCCGAGT33150.021.9373231
GCTACTC56550.020.8812731
GGGAGGC181750.020.837288
CCCAAAG139900.020.638231
GGAGGCT150450.020.625999
CCAAAGT126350.020.5143132
CAAAGTG126650.020.465723
AAAGTGC117800.020.0474114
TGGGAGG181300.019.65797
TACTCGG24250.019.595753
CTACTCG24700.019.530242
Produced by FastQC (version 0.11.5)