FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4557222
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT117300.078.0752945
CGAGTAG32550.074.321213
CCGAGTA33850.072.550322
GTAGCTG126450.072.53966
GAGTAGC77700.072.1840064
TAGCTGG152450.062.718437
GCTGGGA273950.062.2875989
TTGGGAG172650.060.509756
AAGTAGC54600.056.3066564
CCAAGTA53400.056.1042482
AAGTGCT140850.055.462245
CAAGTAG52650.055.3836023
CCCGAGT44950.054.7109261
TTTGGGA159900.054.4380235
GCACTTT143550.053.605661
ACTTTGG144350.052.571253
CTACTCG39050.052.377042
TGAGTAG58600.052.094613
CACTTTG147700.052.075622
TACTCGG40300.051.631813
Produced by FastQC (version 0.11.5)