FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4557222
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT118550.079.499545
CGAGTAG32450.078.988133
CCGAGTA32900.078.564272
GAGTAGC78450.075.073544
GTAGCTG127600.073.973916
TTGGGAG177200.066.066816
TAGCTGG152550.064.565487
CAAGTAG50000.063.6471253
GCTGGGA278150.063.1082239
CCCGAGT42750.061.9794541
CCAAGTA53900.061.3127022
GCACTTT140800.059.77891
TTTGGGA156600.058.8957445
ACTTTGG146200.058.1606253
CACTTTG147550.056.6037642
AAGTAGC56350.056.6025964
TGAGTAG57900.055.8334353
AAGTGCT138950.055.806365
CTGAGTA60050.053.3548052
GGAGGCT207900.051.566969
Produced by FastQC (version 0.11.5)