FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5652993
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA23550.053.8315542
CGAGTAG22900.053.44723
GAGTAGC58150.050.0199434
AGTAGCT89150.049.505185
GTAGCTG93800.045.5159266
TTGGGAG134350.042.5494886
CCCGAGT32500.041.946661
TAGCTGG104050.041.8624657
GCTGGGA174800.041.846959
GGGAGGC174700.037.6673288
GGAGGCT142150.037.3277789
TGGGAGG172600.035.4559947
CGGAGGT29650.035.2090957
TACTCGG23450.034.693423
GGAGGTT77500.033.6293458
TCGGGAG31200.032.767656
CTACTCG24650.032.727762
TTTGGGA146550.032.571615
GCACTTT128550.031.3659691
CTCGGGA29650.030.838315
Produced by FastQC (version 0.11.5)