FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4529532
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG24800.062.416933
GAGTAGC57350.060.8869174
AGTAGCT91700.059.4349865
CCGAGTA26550.058.6063422
GTAGCTG99250.054.478496
GCTGGGA192400.052.6507649
TAGCTGG112200.050.308217
TTGGGAG136150.048.967626
GGAGGCT152550.046.959839
CCCGAGT35450.045.7899061
TTTGGGA130050.043.5138855
CTACTCG26850.043.4636462
TACTCGG27150.043.2248463
GCACTTT117000.042.9784131
AAGTGCT115250.042.1675645
GGGAGGC193200.041.290328
GCTACTC66450.040.8221931
TCGGGAG32350.040.727946
ACTTTGG118150.040.21853
CAAGTAG43650.039.9159773
Produced by FastQC (version 0.11.5)