FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4529532
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG24050.068.855183
CCGAGTA25150.067.2752
GAGTAGC58900.063.3196224
AGTAGCT90950.062.6184655
GTAGCTG97250.056.5629966
TTGGGAG144500.055.1579936
GCTGGGA200150.053.4206439
CCCGAGT33050.053.3720251
TAGCTGG112850.051.2959567
CTACTCG28250.048.4238172
GCACTTT116200.048.3909341
TACTCGG29550.048.24273
GGAGGCT162950.047.6766059
TTTGGGA139400.046.277935
AAGTGCT117500.045.221735
GCTACTC65850.045.1557771
ACTTTGG125500.044.633383
GGGAGGC211900.044.1034368
TCGGGAG35650.043.6237376
CACTTTG128300.043.3225982
Produced by FastQC (version 0.11.5)