FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4412425
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG22800.063.1557963
AGTAGCT91850.061.7682275
CCGAGTA24200.061.607772
GAGTAGC58100.060.4730344
GTAGCTG99800.056.8478136
GCTGGGA199250.053.2981039
TTGGGAG131900.052.674526
TAGCTGG112050.051.9179537
CCCGAGT31150.049.309891
TTTGGGA127900.045.4277345
GGAGGCT152300.045.193519
AAGTGCT113700.045.14895
TACTCGG26700.045.032213
GCACTTT113900.044.8884931
CTACTCG26700.043.9700362
GGGAGGC194150.043.424778
GCTACTC62600.042.277011
TCGGGAG32100.042.1668246
ACTTTGG121500.042.0726783
CACTTTG121450.041.8092272
Produced by FastQC (version 0.11.5)