FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4412425
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG22700.067.557943
CCGAGTA22150.066.635062
GAGTAGC53750.064.028314
AGTAGCT88400.060.189365
TTGGGAG144400.059.035346
GTAGCTG94150.056.5899056
GCTGGGA193050.054.8997659
GCACTTT117650.052.997281
CCCGAGT29150.051.8690531
TTTGGGA140000.051.273715
ACTTTGG124450.050.390183
TACTCGG28150.049.618773
CACTTTG129050.048.761392
CTACTCG27200.048.7047772
TAGCTGG113350.048.528787
GGAGGCT157100.046.8847859
GGGAGGC207050.046.7360088
AAGTGCT112300.046.225665
CTTTGGG146950.044.6835754
TGGGAGG202400.044.5726177
Produced by FastQC (version 0.11.5)