FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3888245
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG28000.084.854883
AGTAGCT106100.083.330485
CCGAGTA29950.081.044132
GAGTAGC67400.079.689274
GTAGCTG115950.076.4378056
TTGGGAG154300.068.96516
TAGCTGG139950.065.644557
GCTGGGA251550.065.543869
CAAGTAG43450.065.4528053
GCACTTT123400.064.000831
CCAAGTA47000.062.9843182
TTTGGGA140950.062.471555
CCCGAGT39750.062.236291
AAGTGCT123900.060.9572075
AAGTAGC48800.060.785264
ACTTTGG129200.060.6856883
CACTTTG129450.060.5362242
TGAGTAG49850.057.1940573
GGAGGCT181400.056.9951749
CTGAGTA51750.055.8113482
Produced by FastQC (version 0.11.5)