FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3888245
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT107500.088.139265
GAGTAGC68950.085.3119354
GTAGCTG116650.081.1021966
CGAGTAG31400.079.794253
CCGAGTA32100.079.399952
TTGGGAG164800.071.5176856
GCTGGGA249900.071.250099
TAGCTGG136850.070.709297
GCACTTT128500.066.676291
CCAAGTA44550.066.2610242
TTTGGGA142800.066.149665
CAAGTAG45350.065.727193
AAGTGCT127100.065.313925
ACTTTGG131650.064.861333
TGAGTAG50150.064.7481843
CCCGAGT40500.064.53261
AAGTAGC48550.063.7677654
CTGAGTA51200.063.4203382
CACTTTG136450.062.2102972
GGAGGCT188500.059.1271869
Produced by FastQC (version 0.11.5)