FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4284541
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG29450.077.007083
AGTAGCT107500.076.013875
GAGTAGC66850.074.634094
CCGAGTA32550.072.576422
GTAGCTG116350.069.7369466
TAGCTGG137600.061.6880157
GCTGGGA238500.061.2188269
TTGGGAG155450.060.7643056
CCCGAGT41950.058.8018461
TTTGGGA141950.053.9649355
CAAGTAG50750.053.482383
GCACTTT130450.053.0791471
AAGTGCT128150.053.034545
CCAAGTA51850.052.645732
ACTTTGG133350.051.8302883
AAGTAGC54850.050.9284554
CTACTCG33400.050.2437632
CACTTTG137150.050.203592
TGAGTAG52850.049.994993
GGAGGCT183750.049.956079
Produced by FastQC (version 0.11.5)