FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4284541
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT104850.082.609215
CGAGTAG28050.081.1101763
CCGAGTA30450.078.7368552
GAGTAGC70700.078.006454
GTAGCTG116350.073.454026
TTGGGAG164450.065.84846
GCTGGGA249750.064.951929
TAGCTGG136100.064.646417
GCACTTT127750.061.8846361
CAAGTAG45950.059.5414283
CCCGAGT40950.058.7264751
AAGTGCT130750.058.7011765
ACTTTGG131750.058.582153
CCAAGTA48800.057.982092
TTTGGGA148550.057.8712585
CACTTTG134650.056.999622
AAGTAGC51000.056.045594
CTGAGTA53900.055.1673852
CTACTCG36600.054.4903762
TACTCGG37800.053.5224043
Produced by FastQC (version 0.11.5)