FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5586291
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT20600.035.299477
GCTGGGA138700.034.467179
TTGGGAG109650.032.9616626
CCGAGTA18250.032.754662
GGAGGTT62050.032.3725178
CTACTCG19850.031.9286122
AGTAGCT73300.031.8240225
CGAGTAG16900.031.0992743
GTAGCTG75950.031.0928216
GGGAGGC136100.030.999428
TACTCGG20550.030.1301083
GCTACTC49800.029.8379541
GGAGGCT117000.029.7833549
TAGCTGG86500.028.3826087
TGGGAGG140550.028.071387
CTCGGGA21600.027.9988125
CCCGAGT27500.027.5415081
GAGTAGC47500.027.2830524
GAGGTTG82650.027.0042979
TCGGGAG23650.026.4851326
Produced by FastQC (version 0.11.5)