FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5586291
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG18250.047.737283
CCGAGTA19850.047.1539422
CTACTCG19600.043.7145582
TACTCGG20850.041.7844283
GAGTAGC45750.040.7609064
CCCGAGT26900.038.5440251
GCTGGGA144350.038.0587589
CGGAGGT24450.037.9889837
TTGGGAG117800.037.4681326
GCTACTC52350.036.9983561
GGAGGCT128650.036.378979
AGTAGCT76000.035.8117075
AGGCGGA34850.034.9155654
TAGCTGG85300.034.7772337
GTAGCTG79450.034.3472636
GGGAGGC150750.034.3414348
CTCGGGA25400.033.73345
TGGGAGG148800.032.3236477
GGCGGAG38100.032.1270455
GGAGGTT65950.031.770578
Produced by FastQC (version 0.11.5)