FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5142861
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT97100.061.8390925
CGAGTAG26450.059.612143
GAGTAGC61150.058.1629944
CCGAGTA28200.057.4619832
GTAGCTG104050.056.3938566
TTGGGAG146850.051.6262636
GCTGGGA212500.051.4653749
TAGCTGG119800.050.121677
CCCGAGT34750.048.155291
CTACTCG28600.047.3411752
TACTCGG30450.044.4515383
GGAGGCT165400.043.355099
AAGTGCT125850.042.849415
GGGAGGC211900.042.1315088
GCTACTC69500.041.8245281
TTTGGGA145950.041.7332845
TCGGGAG34050.041.6548356
GCACTTT133100.040.8605421
CAAGTAG50750.040.290123
CCAAGTA56700.038.867412
Produced by FastQC (version 0.11.5)