FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5142861
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG24250.065.020963
GAGTAGC61900.063.7398264
AGTAGCT94200.063.2874075
CCGAGTA26600.059.27722
TTGGGAG156700.058.078576
GTAGCTG104350.056.648526
GCTGGGA216600.054.5169689
TAGCTGG122250.050.4742477
GCACTTT134950.050.045821
TTTGGGA158150.048.395165
GGAGGCT173650.046.9372839
GGGAGGC226350.046.855458
CCCGAGT34950.046.764531
AAGTGCT127600.045.93165
CACTTTG147200.045.390542
ACTTTGG147200.045.1944543
TGAGTAG51900.044.253163
GCTACTC72900.044.247461
TGGGAGG222050.043.8393177
TCGGGAG36600.042.8857776
Produced by FastQC (version 0.11.5)