FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5080542
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGC45200.041.5736474
TACTCGG18900.041.14133
TTGGGAG113650.041.05086
CTACTCG18250.040.6472782
AGTAGCT72850.040.1247675
CGGAGGT23950.039.9817857
CGAGTAG18150.039.667923
GCTACTC47500.037.952691
GTAGCTG75000.037.8225676
GCTGGGA147450.037.3536229
CCGAGTA19200.037.1356542
TAGCTGG82550.036.892617
GGAGGCT123000.035.530369
GGGAGGC152050.034.4242178
GGAGGTT62450.034.3557858
CTCGGGA24150.033.6881685
AGGCGGA35850.032.3335044
TCGGGAG26600.032.2093056
TGGGAGG151300.031.406617
GGCGGAG36800.031.3031625
Produced by FastQC (version 0.11.5)