FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5080542
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGAG120400.044.9104166
GCTGGGA148950.043.552989
AGTAGCT70500.043.5064935
CGAGTAG15700.042.6497883
CCGAGTA16850.040.593582
GAGTAGC41550.039.8556984
GGAGGCT129550.039.62659
GTAGCTG78250.039.5655986
CTACTCG19900.039.4372862
GGGAGGC153900.039.391918
TAGCTGG84900.038.5018657
TACTCGG20200.038.138713
CGGAGGT25350.038.059277
GCTACTC50050.037.6911471
TGGGAGG155150.037.1254547
GCACTTT111950.035.373721
TTTGGGA130000.034.8923955
CCCGAGT23700.034.6336821
GGAGGTT70300.034.310198
GAGGTTG82450.032.0485959
Produced by FastQC (version 0.11.5)