FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4624123
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG23400.059.382453
CCGAGTA24900.057.268892
AGTAGCT86750.057.1829345
GAGTAGC55250.056.9463464
GTAGCTG95050.053.4015276
TTGGGAG132650.051.0196276
GCTGGGA192350.050.6807639
TAGCTGG107250.048.870957
CCCGAGT31900.048.6016041
CTACTCG26300.048.195872
TCGGGAG32000.046.5733036
GCTACTC61150.045.6370281
TACTCGG27300.045.6247143
GGAGGCT151150.044.2511189
CTCGGGA31850.043.6279225
TTTGGGA129300.042.318645
AAGTGCT111500.042.1007085
GCACTTT115550.041.0637661
GGGAGGC192350.041.061158
CACTTTG119350.038.9215552
Produced by FastQC (version 0.11.5)