FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4553170
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT107850.070.76175
CGAGTAG29250.070.150633
GAGTAGC67800.069.023384
CCGAGTA30500.066.356892
GTAGCTG113450.066.2534566
TTGGGAG156650.059.8402186
TAGCTGG131450.059.26247
GCTGGGA239950.058.9295739
CAAGTAG47850.054.3172573
TTTGGGA148250.052.206715
GCACTTT133400.051.087991
CCCGAGT40500.050.9276771
CCAAGTA52250.049.9001462
ACTTTGG135700.049.4479873
AAGTGCT130650.049.375465
CACTTTG136950.049.2258572
GGAGGCT179000.049.1105969
TGAGTAG55250.048.47563
AAGTAGC53750.048.3549844
GCTACTC75200.048.142511
Produced by FastQC (version 0.11.5)