FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4835717
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG27150.064.174183
CCGAGTA27850.063.6162952
AGTAGCT90750.062.2784465
GAGTAGC60400.059.6002124
GTAGCTG99700.056.1100466
GCTGGGA202300.051.568839
CCCGAGT35550.050.9170761
TTGGGAG143300.050.49346
TAGCTGG116450.050.3886647
TACTCGG28350.049.013873
CTACTCG27900.047.239512
GGAGGCT156800.044.998169
TCGGGAG33350.043.392536
GCTACTC67150.043.1727141
TTTGGGA143950.043.0631945
CGGAGGT34650.042.595667
GCACTTT128250.041.385651
GGGAGGC209200.041.160848
AAGTGCT123250.040.540335
CAAGTAG44500.039.638833
Produced by FastQC (version 0.11.5)