FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4835717
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG24100.066.9214253
CCGAGTA24950.065.21872
AGTAGCT95350.065.0154655
GAGTAGC58800.064.530874
GTAGCTG102000.058.6590586
TTGGGAG148550.055.254346
GCTGGGA208750.054.255159
TAGCTGG117200.053.2015767
CCCGAGT33050.052.720331
GCACTTT124950.049.4407731
CTACTCG31500.047.08592
TTTGGGA147800.046.9122285
TACTCGG32450.046.8168263
ACTTTGG133550.046.149193
GGAGGCT169150.045.9716879
GGGAGGC216200.045.8244178
CACTTTG135550.044.93712
AAGTGCT126850.044.3864565
GCTACTC69050.044.2116131
CAAGTAG47400.043.291313
Produced by FastQC (version 0.11.5)