FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4417020
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG24150.069.760723
CCGAGTA25400.068.048352
AGTAGCT91450.067.705965
GAGTAGC60750.063.641474
GTAGCTG98850.061.836266
GCTGGGA201900.055.2366529
TAGCTGG112950.054.563197
TTGGGAG140100.051.800586
CCCGAGT35100.051.3558121
GGAGGCT150950.046.6940779
AAGTGCT122850.045.4191255
CAAGTAG44400.045.4032823
AAGTAGC48250.044.466324
CGGAGGT30650.044.3959667
CCAAGTA48600.043.8627172
TACTCGG26650.043.7653123
GCTACTC62300.043.748231
CTACTCG26950.043.290872
TGAGTAG50250.042.6965143
GGGAGGC199250.042.637918
Produced by FastQC (version 0.11.5)