FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5407336
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG18750.039.5499843
CCGAGTA19500.039.1502272
GAGTAGC44850.037.723834
TTGGGAG101350.037.2945986
AGTAGCT75600.036.1886335
GCTGGGA145400.036.0476849
CTACTCG18900.034.6770862
GGAGGCT116500.034.1750839
TAGCTGG83100.033.442347
TACTCGG19750.033.1729933
CCCGAGT28300.033.1328771
GTAGCTG78800.032.8917276
GGGAGGC136700.032.8644568
CTCGGGA21700.030.8555725
GCTACTC49750.030.5908551
TGGGAGG139750.030.4986297
TCGGGAG24950.028.5676846
GGAGGTT60650.028.4898918
CGGAGGT21550.028.3976237
TCGCGTT2456.900336E-826.4476325
Produced by FastQC (version 0.11.5)