FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5669026
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG19250.040.767243
CCGAGTA21000.038.4103552
GAGTAGC50500.035.3570334
GCTGGGA160550.032.915569
AGTAGCT82900.032.7417835
TACTCGG22350.031.8913673
CCCGAGT31950.031.3858321
GTAGCTG87350.030.9089246
TAGCTGG93050.030.8725157
CTACTCG20550.030.1395112
GGAGGCT130350.029.384359
TTGGGAG116200.029.3689026
GGGAGGC152050.028.5052998
GCTACTC52600.028.3905831
TCGCGTT2553.263267E-928.234195
CTCGGGA26900.027.3000455
CGGAGGT24800.026.9989437
TCGGGAG30200.025.7473356
CCCAAAG141050.024.9595321
GGAGGTT67650.024.690838
Produced by FastQC (version 0.11.5)