FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5113583
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG16950.042.901873
CCGAGTA18600.039.4835362
AGTAGCT69750.035.1993565
CTACTCG19800.034.5451622
TACTCGG20850.034.5317423
GAGTAGC45150.034.4445534
GCTGGGA141200.033.807749
TTGGGAG103000.033.1333736
TAGCTGG80500.032.377057
GTAGCTG76900.031.8329686
CCCGAGT27400.031.008551
GGAGGCT118150.030.1653799
GCTACTC47100.029.657251
CGGAGGT23600.029.5926787
GGGAGGC140800.028.5335678
CTCGGGA26400.028.0903975
GGAGGTT61550.027.4893448
TCGGGAG28650.027.392176
CCCAAAG123250.026.6980341
TGGGAGG141150.025.8103687
Produced by FastQC (version 0.11.5)