FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6724284
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG25150.038.36043
CCGAGTA25400.038.281382
GAGTAGC60050.035.6094
AGTAGCT93850.034.6753235
GCTGGGA178400.033.3350839
TTGGGAG129450.033.2038656
GTAGCTG99900.031.926746
CTACTCG23050.031.5600722
CTCGCGT3750.030.7188594
TACTCGG23850.030.4894313
CCCGAGT38650.030.2358571
TAGCTGG109200.029.9988847
GGAGGCT149450.029.9165849
CGGAGGT29350.029.6820327
GGGAGGC172400.029.358548
TCGCGTT2802.8558134E-1028.2846625
GCTACTC58400.027.0512941
GGAGGTT76050.026.1292068
TGGGAGG177100.026.0995377
AGGCGGA38800.024.6794954
Produced by FastQC (version 0.11.5)