FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6724284
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA23900.043.6814582
CGAGTAG22050.043.4280243
GCTGGGA178750.040.0796059
TACTCGG27350.038.9611173
GAGTAGC56400.037.9144134
CCCGAGT37100.037.0682951
TTGGGAG139350.036.99476
CTACTCG27650.036.455242
AGTAGCT93950.036.4024245
GGAGGCT151250.034.370699
TAGCTGG103350.034.2061737
GTAGCTG98550.033.75356
GGGAGGC181350.033.5087628
CGGAGGT28700.032.3624427
CTCGGGA32550.032.0738075
TCGGGAG34250.031.7431376
TGGGAGG191200.030.1255937
GGAGGTT80500.028.6211818
GTGCTGG133350.028.5624947
TCGCGTT3153.8198777E-1127.4286335
Produced by FastQC (version 0.11.5)