FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6194686
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA23600.051.25412
CGAGTAG23050.050.602493
GAGTAGC59500.044.651624
TTGGGAG148450.043.6027956
AGTAGCT95100.042.8519675
GCTGGGA191950.042.799229
CTACTCG25400.041.669182
CCCGAGT32750.041.112341
GTAGCTG99850.040.813446
TACTCGG26000.040.4303323
TCGCGTT2900.039.724355
GGGAGGC193800.038.5636758
TAGCTGG113150.038.1159827
GGAGGCT154850.037.0582929
TGGGAGG198950.035.321637
TCGGGAG32200.034.8821756
CGGAGGT30850.034.0747387
GCTACTC67300.033.593591
GGAGGTT83500.033.1977848
CTCGGGA31700.032.933935
Produced by FastQC (version 0.11.5)