FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5818327
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG21900.046.025893
CCGAGTA22850.046.015132
AGTAGCT83750.040.7485165
TTGGGAG123050.040.5480466
GAGTAGC55600.039.6246034
GCTGGGA163300.037.652179
GGAGGCT136050.037.2556729
CCCGAGT32750.037.2134251
GTAGCTG90500.037.1523746
GGGAGGC158850.036.4407358
CTACTCG23650.035.6277772
TAGCTGG101050.035.4110157
TACTCGG22400.035.3559843
CGGAGGT26400.033.8170747
TGGGAGG162300.033.3593527
CTCGGGA25550.032.969575
TCGGGAG27450.032.5235256
GGAGGTT67600.032.164638
GCTACTC58150.031.8718781
TCGCGTT1851.7494312E-731.1341175
Produced by FastQC (version 0.11.5)