FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5818327
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA21700.052.0915152
CGAGTAG22050.048.978983
AGTAGCT82750.044.2008135
TTGGGAG132550.043.781426
GAGTAGC54250.043.3985374
CGGAGGT29800.042.0404667
GCTGGGA165150.041.8098229
CCCGAGT32150.040.7598341
GTAGCTG87650.040.579776
GGAGGCT146550.039.550179
TACTCGG25500.038.964223
CTACTCG25350.037.7746772
GGGAGGC181350.037.598198
GCTACTC63200.037.0261271
TAGCTGG98650.036.9307377
TCGGGAG30350.036.0594756
GGAGGTT72550.035.8264968
TCGCGTT2252.1827873E-1135.2001655
CTCGGGA28200.034.4682465
TGGGAGG181650.033.730967
Produced by FastQC (version 0.11.5)