FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6253872
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA21800.041.2989042
CGAGTAG21550.038.421023
TCGCGTT3100.037.1600765
GCTGGGA160650.036.1668859
AGTAGCT85500.034.6936045
TACTCGG25150.034.0664833
CCCGAGT32850.034.027541
CTACTCG23650.033.804682
TTGGGAG127100.032.9682436
GTAGCTG88250.032.7966656
GAGTAGC54450.032.5278634
GGAGGCT136300.032.2219859
CTCGCGT4300.031.8129164
TAGCTGG97850.031.5656557
GGGAGGC165150.029.2960478
GCTACTC57250.028.4686661
CGCGTTG3900.027.6914046
CTCGGGA28100.027.6716945
TGGGAGG169950.027.3671557
CGGAGGT24950.026.5482317
Produced by FastQC (version 0.11.5)