FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5879468
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA21450.046.671242
CGAGTAG20900.043.404493
AGTAGCT86050.040.997335
GCTGGGA162900.039.821189
GAGTAGC54250.039.150084
TAGCTGG97000.038.299087
GTAGCTG93100.037.274156
TTGGGAG123000.037.1103066
TCGCGTT2151.2732926E-1136.835335
CCCGAGT34250.035.80911
TACTCGG23100.034.283973
GGAGGCT134750.033.6606259
CGGAGGT24900.033.5404627
GGGAGGC157000.033.0632868
CTACTCG21600.032.3429642
TGGGAGG158000.031.6237057
GCTACTC55600.030.103591
GGAGGTT67750.029.2236048
TCGGGAG27850.028.9536446
AGGCGGA36950.028.6426564
Produced by FastQC (version 0.11.5)