FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6210389
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT3350.045.1342745
TACTCGG22200.039.2431953
CGAGTAG22150.037.70653
CCGAGTA23900.037.0543442
GCTGGGA163200.037.0149579
CTACTCG22450.036.8819162
GAGTAGC55950.036.2894974
TTGGGAG124600.036.1733136
AGTAGCT86400.035.8332865
CGCGTTG4050.035.555516
GGAGGCT138900.034.6265879
GTAGCTG90200.034.323686
GGGAGGC165950.033.6245468
TAGCTGG97300.033.447037
TCGGGAG29050.032.468126
GCTACTC55900.032.4573971
CTCGGGA28000.032.399965
CGGAGGT28550.032.0279857
TGGGAGG167050.031.1187887
AGGCGGA38150.030.0078264
Produced by FastQC (version 0.11.5)