FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5553258
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA20950.042.6306952
CGAGTAG19300.039.542593
GAGTAGC50400.039.4271284
AGTAGCT77650.038.7571145
GCTGGGA152250.038.3040169
TTGGGAG112800.037.1475756
GTAGCTG81450.035.8881876
TAGCTGG88950.035.4523287
CGGAGGT23450.034.6938257
CCCGAGT29300.033.9745671
GGAGGCT121050.032.653089
CTACTCG19000.032.6009032
TACTCGG20250.031.9988253
GGGAGGC152550.031.8100438
TGGGAGG149900.029.5866417
GGAGGTT65000.029.352778
TCGGGAG28100.028.9526776
CTCGGGA26450.028.5811845
GCTACTC47900.028.161031
TCGCGTT2054.298472E-728.0965335
Produced by FastQC (version 0.11.5)