FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5553258
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG17350.044.818123
CCGAGTA19400.044.1646232
TTGGGAG118350.041.186386
AGTAGCT74450.040.1343845
GCTGGGA157400.039.386149
GAGTAGC45600.038.9997144
TCGCGTT3000.038.4000635
GGAGGCT127200.037.0765349
GGGAGGC152300.036.685558
CGGAGGT27900.036.3871577
GTAGCTG80500.035.86596
TACTCGG21300.035.8307233
CCCGAGT27800.035.741071
CTACTCG20550.034.685882
TAGCTGG92150.033.8318527
GCTACTC53750.032.5507551
TGGGAGG162250.032.394517
CTCGGGA23200.032.2759175
GGAGGTT69050.031.9073688
TCGGGAG26600.031.1278746
Produced by FastQC (version 0.11.5)