FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5550834
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA26750.060.2915842
CGAGTAG26000.060.0923043
AGTAGCT100300.055.705385
GAGTAGC61900.054.9014474
GCTGGGA224600.050.490219
GTAGCTG110250.050.3514676
TTGGGAG154150.049.6040766
TAGCTGG125950.046.7046627
TACTCGG29850.046.5527573
CTACTCG30100.045.68772
CCCGAGT38750.044.221931
GGAGGCT180500.042.4424829
GCACTTT128500.042.3595281
GGGAGGC227850.041.1432238
TTTGGGA151250.040.986815
TCGGGAG39950.040.0103656
AAGTGCT139600.039.6622475
TGGGAGG224400.037.9575547
ACTTTGG140750.037.90553
CACTTTG145300.037.1644862
Produced by FastQC (version 0.11.5)