FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6406494
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG23450.051.2743233
CCGAGTA25200.047.999692
GAGTAGC60550.041.736814
GCTGGGA185150.041.0651369
AGTAGCT97400.040.9521875
GTAGCTG100350.039.748316
TAGCTGG109850.038.866997
CCCGAGT35600.037.6189081
TACTCGG25350.037.490593
CTACTCG24900.036.7226562
TTGGGAG135400.036.3185356
GGAGGCT150550.035.2468079
GCTACTC61450.033.7453461
GGGAGGC182950.032.270658
CGGAGGT31650.030.255497
CTCGGGA30800.029.9216485
CCCGAAT14200.029.9162331
TGGGAGG180650.029.8916287
TCGGGAG32200.029.7387056
GGAGGTT80300.029.6782788
Produced by FastQC (version 0.11.5)