FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6493567
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG24350.047.6032683
CCGAGTA25650.047.171782
AGTAGCT98900.042.367865
GAGTAGC61550.040.47234
GCTGGGA198600.040.2033429
GTAGCTG108400.038.3227236
TAGCTGG117100.037.6888967
TTGGGAG147000.036.7327776
CCCGAGT38750.036.668031
GGAGGCT160450.034.147179
CGGAGGT28850.031.9428757
GGGAGGC199300.031.717368
CTACTCG27600.031.0526182
TGGGAGG192650.030.5699087
TACTCGG28650.030.1554933
GGAGGTT78550.029.238458
AGGCGGA41300.029.1122824
GCTACTC67150.028.3563921
GGCGGAG44100.027.9169125
GTGCTGG135850.027.876357
Produced by FastQC (version 0.11.5)