FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5745015
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG19900.053.5455553
CCGAGTA21950.050.5285762
GAGTAGC53850.043.051174
AGTAGCT86350.042.773015
TCGCGTT2250.041.59835
CCCGAGT33350.040.662141
GCTGGGA172150.039.772999
GTAGCTG91950.038.8369146
GGAGGCT140850.038.490449
TTGGGAG132450.037.9961856
TAGCTGG99000.037.5984657
TACTCGG23250.037.469443
CTACTCG23650.037.4562952
GGGAGGC172900.034.5619248
GCTACTC61200.033.119451
TGGGAGG176800.031.4376327
CGGAGGT26350.031.1486347
TCGGGAG32300.030.983266
CTCGGGA29100.030.1843365
GGAGGTT70950.029.6309268
Produced by FastQC (version 0.11.5)