FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5745015
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA22000.051.0536272
CGAGTAG23000.049.1469423
GAGTAGC56950.047.6620064
AGTAGCT85850.047.132945
CTACTCG22600.043.9638142
GTAGCTG91700.042.948356
TACTCGG23200.042.516483
TAGCTGG99150.042.2628487
GCTGGGA178900.042.2183469
TTGGGAG132450.042.0743566
CCCGAGT31700.039.7472651
GGAGGCT144950.037.7512559
GGGAGGC181050.037.4213648
TCGGGAG30600.035.529086
CTCGGGA29050.034.6984755
TGGGAGG180250.034.1921927
GCTACTC58200.033.772881
CGGAGGT30550.032.7591067
GCACTTT124150.031.5486431
TTTGGGA144350.031.2736955
Produced by FastQC (version 0.11.5)