FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6668748
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG24750.041.307323
CCGAGTA28350.038.868882
GAGTAGC63350.035.1176724
AGTAGCT98850.034.8148965
CTACTCG25200.034.581872
GCTGGGA187150.033.8152739
TAGCTGG111750.032.7287987
TACTCGG26400.032.725873
TTGGGAG136400.032.4619526
GTAGCTG104900.032.1892786
GCTACTC63600.031.6412011
CCCGAGT41450.030.8002741
GGAGGCT160650.029.6233799
GGGAGGC184300.028.4393658
TCGCGTT2303.738387E-828.1727035
CTCGCGT2955.2386895E-1026.8463064
TGGGAGG186000.026.4762827
TCGGGAG32600.026.281086
GGAGGTT76450.026.086528
CTCGGGA32050.025.6088855
Produced by FastQC (version 0.11.5)