FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6780034
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG21200.048.5647283
CCGAGTA23700.046.4797632
TACTCGG28300.042.74093
CTACTCG27750.040.734042
TTGGGAG149600.039.9460336
TCGCGTT3250.039.8764345
AGTAGCT96900.038.042885
GCTGGGA195650.037.9413229
GAGTAGC58100.037.3001984
CGGAGGT34650.036.3631947
GGAGGCT171750.035.717129
CCCGAGT37150.035.6601371
GTAGCTG105550.035.1980556
CTCGGGA31950.034.25315
GGGAGGC206100.034.0257878
GCTACTC66850.033.926041
TAGCTGG113600.033.4017
TCGGGAG36950.032.1511656
TGGGAGG204150.031.5646427
GGAGGTT88350.031.4563228
Produced by FastQC (version 0.11.5)