FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6578265
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG25200.045.713143
CCGAGTA26650.044.5916522
GAGTAGC61300.040.873364
AGTAGCT94900.040.8166775
GCTGGGA188100.039.5779539
TTGGGAG145300.037.8572736
GTAGCTG103200.037.8130046
TAGCTGG114750.036.704967
CCCGAGT36900.036.5485571
GGAGGCT155950.034.856459
CGGAGGT32150.033.8156367
TCGCGTT2353.6379788E-1133.7012835
TACTCGG27900.033.2894863
CTACTCG27800.032.9022642
GGGAGGC193050.032.6332138
GGAGGTT82850.031.3715748
GCTACTC66800.031.0936051
TCGGGAG34900.029.9132866
CTCGGGA32550.029.8615
TGGGAGG195550.029.8596427
Produced by FastQC (version 0.11.5)