FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6578265
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA27050.049.507982
GAGTAGC63100.047.9236034
AGTAGCT102500.045.096625
CGAGTAG27800.045.0644383
GTAGCTG106350.042.6516536
GCTGGGA199000.041.535719
TAGCTGG113350.040.5893557
TTGGGAG146600.039.634416
CCCGAGT37550.039.1161731
GGAGGCT159500.037.060849
TACTCGG27600.035.2171483
GGGAGGC195150.035.0868848
CGGAGGT34550.034.8017657
GGAGGTT82250.033.8772288
CTACTCG27300.033.230542
TGGGAGG197850.032.0242847
GCACTTT135450.031.5218561
GCTACTC65100.030.4150031
TTTGGGA157750.030.0323525
AGGCGGA46000.029.4258844
Produced by FastQC (version 0.11.5)