FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6157193
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT2500.043.1985785
CCGAGTA22300.038.7563362
CGAGTAG22000.038.2896463
AGTAGCT84550.035.5091825
TTGGGAG126650.034.7908026
GAGTAGC52350.034.382824
GCTGGGA165500.033.1058589
GTAGCTG90750.032.3691836
GGAGGCT136800.030.68329
CTCGCGT4700.030.6372874
TAGCTGG99300.030.597187
CGGAGGT26800.030.357217
TACTCGG23250.030.3473873
GGGAGGC161300.029.5489338
CTACTCG22950.029.4992872
CCCGAGT35700.029.0916161
TGGGAGG169500.028.204387
GGAGGTT71200.027.2013518
GCTACTC58100.027.1857661
TCGGGAG30200.027.177916
Produced by FastQC (version 0.11.5)