FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6157193
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT2300.050.0866935
CGAGTAG21600.045.999763
CCGAGTA22700.045.0394132
GAGTAGC54650.039.2605484
AGTAGCT89100.038.8684845
GCTGGGA173100.037.5599029
TACTCGG24300.036.4442563
GTAGCTG95800.036.074976
CTACTCG23850.035.9243432
TTGGGAG126550.035.786466
CCCGAGT32100.035.6642381
TAGCTGG103100.035.266557
GCTACTC56200.033.950831
GGAGGCT144850.033.0052769
CGGAGGT29000.032.7722437
GGGAGGC172650.031.2769898
GGAGGTT78050.028.5046588
CGCGTTG4050.028.4442966
TGGGAGG173250.028.342717
GCACTTT122350.027.8942831
Produced by FastQC (version 0.11.5)