FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6587817
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT2200.058.9074365
CGAGTAG21900.037.807453
CCGAGTA24000.037.2136462
CTCGCGT3750.036.4789734
CGGAGGT27250.036.197157
AGTAGCT91250.034.3223275
GCTGGGA174300.033.912999
AGGCGGA37100.033.379124
GTAGCTG95650.033.044556
GAGTAGC56550.032.9752044
TAGCTGG103500.032.4860467
TACTCGG19700.031.7963033
CGCGTTG4100.031.6088686
GGCGGAG42150.030.4048425
TTGGGAG126650.030.1294366
CCCGAGT34000.029.6978451
GGAGGCT144950.029.6535329
GGAGGTT74300.029.0705188
CTACTCG19900.028.955342
GAGGTTG91000.027.9288889
Produced by FastQC (version 0.11.5)