FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5218872
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG17100.039.9997523
CCGAGTA18900.038.476322
AGTAGCT71350.035.924445
TTGGGAG114450.035.8585746
TACTCGG20500.035.4729543
GAGTAGC45350.035.404414
GCTGGGA149100.035.1553849
GGAGGCT124850.034.7173089
CTACTCG19950.034.285832
GTAGCTG78400.032.2348026
GGGAGGC149250.031.9357858
CCCGAGT29050.031.229621
TAGCTGG87200.029.8074427
TCGCGTT3151.8189894E-1229.7143845
GCTACTC52900.029.5356391
CGGAGGT24450.029.1534737
TGGGAGG152800.028.8849147
CTCGGGA25950.028.0232165
TCGGGAG27500.026.9673656
GCACTTT102250.026.4064641
Produced by FastQC (version 0.11.5)