FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4032894
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG15750.061.255993
CCGAGTA16400.059.7061962
AGTAGCT65450.050.4931835
GTAGCTG68750.048.80266
GCTGGGA139800.048.463819
GAGTAGC41900.048.1136554
CCCGAGT22950.046.745381
TAGCTGG78500.044.5755127
TTGGGAG104250.043.9939656
TACTCGG17550.043.4863623
TCGCGTT1505.7480065E-1043.1997225
CTACTCG17950.042.1162032
GGAGGCT113200.041.406619
GGGAGGC140700.039.607428
TCGGGAG23100.038.025736
GCTACTC45850.036.9031681
AAGTGCT92850.036.445655
TGGGAGG141150.036.3185777
CGGAGGT22650.036.2381827
CCCAAAG125050.034.0281941
Produced by FastQC (version 0.11.5)